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藥靶細(xì)胞株 > 腫瘤免疫細(xì)胞株 > CBP74192hIL2/IL15 Dual Effector(hCD25/hCD122/hCD132) Repor

hIL2/IL15 Dual Effector(hCD25/hCD122/hCD132) Repor
名稱 hIL2/IL15 Dual Effector(hCD25/hCD122/hCD132) Repor
型號(hào) CBP74192
報(bào)價(jià)
特點(diǎn) hIL2/IL15 Dual Effector(hCD25/hCD122/hCD132) Reporter Cell
  • 詳細(xì)內(nèi)容
CBP74192
I. Background

The particles contain a firefly luciferase gene driven by the human IL-2 promoter. After transduction, activation of the IL-2 signaling pathway in the target cells can be monitored by measuring the luciferase activity.

IL-15 has been shown to be produced by human fetal astrocytes and microglial cells in response to IL-1beta, IFN-gamma, or TNF- alpha and may thus play a role in T-cell mediated immune responses in the human central nervous system. IL-15 stimulates proliferation of the established T-cell line CTLL-2, CD8(+) memory T- cells require IL-15 for proliferation. IL-15 is also able to induce generation of cytolytic cells and LAK cells activity in vitro. IL-15 appears to function as a specific maturation factor for NK-cells and can mimic the bone marrow microenvironment in vitro, which is required for NK- cells to acquire lytic functions. IL-15 induces proliferation of mast cells in the absence of functional IL-2 receptor components and utilizes a receptor of 60-65 kDa that is distinct from known components of T-cell IL-15 receptors. The IL-15 receptor on mast cells recruits JAK2 and STAT5, instead of JAK1, JAK3, and STAT3 and STAT5 that are activated in T-cells. The alpha subunit of the IL-15 receptor (IL-15RA) is structurally related to the alpha subunit of the IL-2 receptor and confers high affinity binding of IL-15 to its receptor. IL-15 binds to the beta and gamma chains of the IL2 receptor, which are known to be required for ligand internalization and signal transduction.

II. Introduction
Expressed gene:IL2R、IL15R
Stability:32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium:90% FBS+10% DMSO
Culture Medium:RPMI-1640+10%FBS+1ug/ml puromycin+800ug/ml hygromycin+10ug/ml blasticidin
Mycoplasma Testing:Negative
Storage:Liquid nitrogen
Application(s):Functional(Report Gene) Assay

III. Representative Data

CBP74192 流式1新.jpg

Figure 1. Recombinant hIL2/IL15 Dual Effector(hCD25/hCD122/hCD132) Reporter Cell constitutively expressing IL2Rα.

CBP74192 流式2新.jpg

Figure 2. Recombinant hIL2/IL15 Dual Effector(hCD25/hCD122/hCD132) Reporter Cell constitutively expressing IL2Rβ.


CBP74192 流式3新.jpg

Figure 3. Recombinant hIL2/IL15 Dual Effector(hCD25/hCD122/hCD132) Reporter Cell constitutively expressing IL2Rγ.

CBP74192.jpg

Figure 4. Dose Response of Recombinant Human IL2/IL15 in hIL2/IL15 Dual Effector(hCD25/hCD122/hCD132)Reporter Cells (C26).




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