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聯(lián)系人：蔣經(jīng)理
電話：4008750250
號(hào)碼：
手機(jī)：18066071954
地址：南京市棲霞區(qū)緯地路9號(hào)
Email： zhangxiangwen@cobioer.com

名稱	c-Kit W557_K558del-D816V/BaF3
型號(hào)	CBP73285
報(bào)價(jià)
特點(diǎn)	c-Kit W557_K558del-D816V/BaF3,母細(xì)胞：BaF3，KIT W557_K558del 導(dǎo)致 Kit 蛋白的近膜結(jié)構(gòu)域（外顯子 11）中從氨基酸 557 到 558（PMID：12918066）的 2 個(gè)氨基酸缺失。 W557_K558del 導(dǎo)致 Kit 的組成型激活和 Erk 磷酸化增加，并導(dǎo)致細(xì)胞培養(yǎng)中 ETV1 的侵襲性和表達(dá)增加。

CBP73285
I. Introduction
Cell Line Name：	c-Kit W557_K558del-D816V/BaF3
Host Cell:	Ba/F3
Stability:	16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Application:	Anti-proliferation assay and PD assay
Freeze Medium:	90% FBS+10% DMSO
Complete Culture Medium:	RPMI-1640+10%FBS
Mycoplasma Status:	Negative

II.Background
KIT W557_K558del results in the deletion of 2 amino acids in the juxtamembrane domain (exon 11) of the Kit protein from amino acids 557 to 558 (PMID: 12918066). W557_K558del results in constitutive activation of Kit and increased Erk phosphorylation, and leads to increased invasiveness and expression of ETV1 in cell culture (PMID: 25239608, PMID: 26936919).

III. Representative Data
1. WB of c-Kit W557_K558del-D816V/BaF3
2. Sanger of c-Kit W557_K558del-D816V/BaF3 Figure 2. c-Kit W557_K558del-T670I/BaF3 D816V Figure 3. c-Kit W557_K558del-T670I/BaF3 W557_K558del
3. Anti-proliferation assay Figure 4. CTG Proliferation Assay of BaF3 c-KIT W557_ K558del/D816V (C5).